The 38-year-old full-back has won eight trophies during two long spells covering 17 seasons at the team where he started his career.
He will play his last game with Sevilla on May 25 against Barcelona during the ongoing La Liga 2023-24 season.
“Navas will leave Sevilla at the end of the 2023/24 season the captain told the club, his teammates, and the coaching staff at the training session this morning,” said Sevilla in a statement.
“The club would like to wish Jesús Navas the best as embarks on a new professional chapter, as well as thank him for his commitment, professionalism, and honesty with which he has shown throughout his career and for which he is one of our all-time greatest symbols.
“The club will always open its door to Jesús Navas at any time in the future and Sevilla will continue to always be his home.”
Navas has played 688 games for Sevilla across all competitions, making his 500th appearance in La Liga for the Andalucian side in midweek.
The Spaniard has scored 39 goals and provided 119 assists in his time at Sevilla, starting his career as a winger and later playing at right-back.
Navas has earned four Europa League titles, two Copa del Rey triumphs, the European Super Cup, and the Spanish Super Cup during his time at the club.
In 2013 Navas departed for Manchester City, where he won the Premier League and two League Cups, later returning to Sevilla in 2017.
Navas has won more trophies and played more matches for Sevilla than any other player in the club’s history.
With Spain the defender won the World Cup in 2010, the European Championship in 2012, and the Nations League in 2023.